VALIDATION OF A NEW, ULTRA-FAST BLASTOCYST WARMING TECHNIQUE REDUCES WARMING TIMES TO 1 MIN AND YIELDS SIMILAR SURVIVAL AND RE-EXPANSION COMPARED TO BLASTOCYSTS WARMED USING A STANDARD METHOD

نویسندگان

چکیده

Embryo warming is an essential practice in IVF. During warming, a blastocyst moved through series of solutions to remove the cryopreservation agent. This technique has demonstrated high survival and implantation rates, but also requires approximately 10-15 minutes bench time for embryologist. study introduces new approach embryo deemed ultra-fast which only involves blastocysts thawing solution (TS) at 37°C 1 minute before placing them culture media. method would eliminate need dilution while decreasing number steps complete process. It objective this compare initial re-expansion after 24 hours that undergo versus standard methods. DESIGN: Prospective Randomized. All were warmed using media from Fujifilm. Blastocysts frozen on day 5 donated research randomly utilizing either (1 TS) or TS, 4 dilute solution, 8 washing solution). The embryos evaluated directly hours. A total 71 randomized between (n=39) (n=32). There was no difference procedures: 39/39 (100%) 32/32 (100%; P=NS). After culture, 39 re-expanded, 31 32 (96.9%) re-expanded (P=NS). Our data suggest there are significant differences (after hours) compared

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ژورنال

عنوان ژورنال: Fertility and Sterility

سال: 2021

ISSN: ['0015-0282', '1556-5653']

DOI: https://doi.org/10.1016/j.fertnstert.2021.07.456